Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

Download original image
Fig. 4. DMK inhibits the production of elastase and downregulates the expression of MMP-12 in HaCaT Cells. (A) Diagram depicting the role of MMP-7 and MMP-12 in the degradation of elastin. (B) HaCaT cells (2×107 cells) were exposed to DMK at various times, and the production of elastase was measured by the Human Neutrophil Elastase Simple-Step ELISA Assay Kit (n=3). (C) HaCaT cells (2×106 cells) were exposed to DMK at various times and the mRNA levels of MMP-7 and MMP-12 were measured by real-time RT-PCR (n=4). (D) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the protein expression of MMP-12 was measured by Western blot analysis. **p<0.01 and ***p<0.001 vs Control (DMSO).
Biomolecules & Therapeutics 2024;32:240~248 https://doi.org/10.4062/biomolther.2023.131
© Biomolecules & Therapeutics