Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 3. DMK inhibits the production of collagenase and downregulates the expression of MMP-1, MMP-9, and MMP-10 in HaCaT Cells. (A) Diagram depicting the types and roles of various matrix metalloproteinases in the degradation of collagen. (B) HaCaT cells (5×106 cells) were exposed to DMK at various times, and the production of collagenase was measured by the human collagenase-1 ELISA assay (n=3). (C) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the mRNA levels of MMP-1, MMP-9, and MM-10 were measured by real-time RT-PCR analysis (n=4). (D) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the protein expression of MMP-1, MMP-9, and MMP-10 was measured by Western blot analysis. **p<0.01 and ***p<0.001 vs Control (DMSO).
Biomolecules & Therapeutics 2024;32:240~248 https://doi.org/10.4062/biomolther.2023.131
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