Table 1 Sequences of the real-time RT-PCR primers
| Accession |
Gene | Primer sequence | |
|---|---|---|---|
| Human | NM_001289726.1 | GAPDH | Forward: 5’-GGAGAGTGTTTCCTCGTCCC -3’ Reverse: 5’-ACTGTGCCGTTGAATTTGCC -3’ |
| NM_000917.4 | Proyl-4-Hydroxylase | Forward: 5’ -CCGAGCTACAGTACATGACCC-3 Reverse: 5’-TGGCTCATCTTTCCGTGCAA-3 |
|
| NM_000088.4 | COL1A1 | Forward: 5’-TGCTCGTGGAAATGATGGTG-3 Reverse: 5’-CCTCGCTTTCCTTCCTCTCC-3 |
|
| NM_002421.4 | MMP-1 | Forward: 5’-TGTGGTGTCTCACAGCTTCC-3’ Reverse: 5’-TTGTCCCGATGATCTCCCCT-3’ |
|
| NM_004994.3 | MMP-9 | Forward: 5’-ACGATGACGAGTTGTGGTCC-3’ Reverse: 5’-TCGCTGGTACAGGTCGAGTA-3’ |
|
| NM_002425.3 | MMP-10 | Forward: 5’-AGTTAACAGCAGGGACACCG-3’ Reverse: 5’-GTCTAGGGAAGCCTTGCTCC-3’ |
|
| NM_002423.5 | MMP-7 | Forward: 5’-GGAGTGCCAGATGTTGC-3’ Reverse: 5’-ATCTCCTCCGAGACCTG-3’ |
|
| NM_002426.6 | MMP-12 | Forward: 5’-TTTGGTGGTTTTTGCCCGTG-3’ Reverse: 5’-ATGTCATCAGCAGAGAGGCG-3’ |
|
| NM_005252.4 | c-Fos | Forward: 5’-CCTGCCTCTCCTCAATGACC-3’ Reverse: 5’-TCGGGGTAGGTGAAGACGAA-3’ |
|
| NM_002228.4 | c-Jun | Forward: 5’-TATGACGATGCCCTCAACGC-3’ Reverse: 5’-CTGGATTATCAGGCGCTCCA-3’ |
|
| NM_000575.5 | IL-1α | Forward: 5’-AGTAGCAACCAACGGGAAG-3’ Reverse: 5’-GCCGTGAGTTTCCCAGA-3’ |
|
| NM_000576.3 | IL-1β | Forward: 5’-CTGAGCTCGCCAGTGAAAT-3’ Reverse: 5’-TCGTGCACATAAGCCTCG-3’ |
|
| XM_054341820.1 | IL-1R | Forward: 5’- GGAGACGGAGGACTTGTGTG-3’ Reverse: 5’-ACTGGCCGGTGACATTACAG-3’ |