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Fig. 1. DMK increases the level of 4-hydroxyproline and promotes the synthesis of collagen in HaCaT cells. (A) Diagram depicting the conversion of proline to hydroxyproline by prolyl 4-hydroxylase (P4H). (B) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the mRNA level of P4H was measured by real-time RT-PCR (n=4). (C) HaCaT cells (1×105 cells) were exposed to DMK for 24 h, and the level of 4-hydroxyproline was measured by an immunocytochemical assay. (D) HaCaT cells (1×106 cells) were exposed to DMK at various times, and the production of collagen type 1α1 (COL1A1) was measured by the collagen type 1α1 ELISA assay (n=3). (E) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the mRNA level of COL1A1 was measured by real-time RT-PCR (n=4). *p<0.05, **p<0.01, and ***p<0.001 vs Control (DMSO).
Biomolecules & Therapeutics 2024;32:240~248 https://doi.org/10.4062/biomolther.2023.131
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