Fig. 3. The effect of MA on cell viability and melanin contents of α-MSH (0.2 µM)-treated B16F10 cells. (A) Cell viability was determined by MTT assay. (B) Melanin contents were measured by melanin assay. (C) Melanin is stained dark black with FM stain. Red arrows indicate dendrite formation from melanocytes. Scale bar is 50 µm. (D) Macroscopic view of B16F10 cell pellets. Data are presented as the mean ± SD (n=3, #p<0.05 compared with the control group; ***p<0.001, **p<0.01 and *p<0.05 compared with the α-MSH-treated group).
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