Fig. 2. The effect of MA on cell viability and melanin contents of α-MSH (0.2 µM)-treated MNT-1 cells. α-MSH-untreated cells were used as negative controls, and arbutin-treated cells were used as positive controls. MA (10-150 µg/mL) and arbutin (50 µg/mL) were treated to MNT-1 cells for 72 h. (A) Cell viability was determined by MTT assay. (B) Melanin contents were measured by melanin assay. (C) Melanogenesis was observed under optical microscopy (10×). Scale bar is 100 µm. Data are presented as the mean ± SD (n=3, ##p<0.01 compared with the control group; **p<0.01 and *p<0.05 compared with the α-MSH-treated group).
© Biomolecules & Therapeutics
