Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 6. BTZ induces cell death in undifferentiated SH-SY5Y cells but has no significant effect on TUBB3 level. (A, B) SH-SY5Y cells were treated with 0.1% DMSO or 1, 5, and 20 nM BTZ for 48 h. (A) The representative images present the cell density and morphology of SH-SY5Y cells treated with DMSO and BTZ (20 nM). Scale bar=250 μm. (B) MTT assay was used to measure cell viability. (C-J) SH-SY5Y cells were treated with 0.1% DMSO or 5, 10, and 20 nM BTZ for 36 h. After treatment, total cell lysates were collected, and a western blot analysis was conducted. (C, D) Representative images of protein bands showing (C) TUBB3 and (D) GFAP. Representative images of protein bands with their respective densitometer graph (E, F) BCL-2/BAX ratio, (G, H) p-CREB, and (I, J) BDNF. GAPDH was used as loading controls. CNTF was used as a positive control for GFAP. (K) RT-PCR was performed to quantify Bdnf mRNA levels. Total RNA was extracted from SH-SY5Y cells treated with 0.1% DMSO or 5, 10, and 20 nM BTZ for 24 h. Gapdh was used as an internal control. Data are shown as the mean ± SEM (n=3). *p<0.05, **p<0.01 (Student’s t-test). Images of SH-SY5Y cells treated with DMSO and BTZ are shown in Supplementary Fig. 7. Uncropped images of western blots are shown in Supplementary Fig. 8, 9, and 10.
Biomolecules & Therapeutics 2024;32:65~76 https://doi.org/10.4062/biomolther.2023.134
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