Fig. 3. Time-lapse images of BTZ treated NSCs. BTZ-treated NSCs round up, divide and express neuronal marker protein in the presence of mitogens. (A-C) Time-lapse microscopic photos of BTZ treated NSCs were taken every 20 min. (A, B) A cell marked in a red circle rounded up (A), and divided into two daughter cells (B). One of the daughter cells (C) differentiated into a neuron as determined by immunocytochemistry using TuJ1 (D, Green). Timestamp indicates the elapsed time from BTZ treatment. Scale bar=100 μm.
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