Fig. 4. Interference of cell cycle progression by domperidone. TNBC BT-549 and CAL-51 cells were treated with domperidone (0, 10, 20, and 50 µM) for 24 h. (A) Cell cycle analysis by flow cytometry with PI staining. (B) The immunoblot analysis of cell cycle related proteins cyclins A, B, D1, D2, D3, and E, and CDK1, 2, and 4. β-Actin was used as the loading control. (C) The immunoblot analysis of CDK inhibitors p16, p21, and p27, and p53.
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