Fig. 3. Induction of mitochondrial ROS by domperidone. (A) TNBC BT-549 and CAL-51 cells were treated with domperidone (0, 10, 20, and 50 µM) for 24 h and the levels of mitochondrial superoxide were analyzed by flow cytometry with MitoSOXTM Red staining. The bar graphs represent the mean ± SD (n=3). *p<0.05 and **p<0.01 compared to the control group. (B) TNBC cells were pretreated with 100 nM of Mito-TEMPO for 3 h before domperidone treatment (100 µM for 24 h). The cell viability was measured by WST assay. **p<0.01 compared to the domperidone treated group.
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