Fig. 1. Antiproliferative effect of domperidone in TNBC cells. (A) TNBC BT-549 and CAL-51 cells were treated with domperidone (0, 5, 10, 20, 50, and 100 µM) for 24 (black) and 48 h (gray) and cell viability was determined by WST cell viability assay. The data are shown as the mean ± SD (n=4). **p<0.01 compared to the control group (0 µM domperidone). (B) BT-549 and CAL-51 cells treated with varying concentrations of domperidone (0, 10, 20, and 50 µM) for 24 h were analyzed by flow cytometry with annexin V/PI staining. (C) The level of cleaved forms of caspase-3, -7, -8, and -9, and PARP were monitored by immunoblot. β-Actin was used as the loading control.
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