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Fig. 5. LicoD alters the expression of proteins associated with AKT signaling in EGF- or TPA-induced JB6 cells. (A) The effect of LicoD on the expression of AKT signaling-related proteins in EGF-induced JB6 cells was investigated. JB6 cells were treated with EGF with 0, 2.5, 5, and 10 μM LicoD and then incubated for 30 min. The cell lysates were performed to western blotting using p-AKT, panAKT, p-GSK3β, GSK3β, p-NFκB, NFκB, p-mTOR and mTOR antibodies. β-actin was used as a loading control. (B) The relative expression of p-AKT, p-mTOR, p-GSK3β and p-NFκB in EGF-induced JB6 cells was shown after normalization against panAKT, mTOR, GSK3β, and NFκB, respectively. (C) The effect of LicoD on the expression of AKT signaling-related proteins in TPA-induced JB6 cells was examined. JB6 cells were treated with TPA with 0, 2.5, 5, and 10 μM LicoD and then incubated for 60 min. (D) The relative expression of p-AKT, p-mTOR, p-GSK3β and p-NFκB in TPA-induced JB6 cells was shown after normalization against panAKT, mTOR, GSK3β, and NFκB, respectively. Significantly different at: #p<0.05, ##p<0.01, and ###p<0.001 compared to the control; *p<0.05, **p<0.01, and ***p<0.001 compared to group treated with EGF or TPA only.
Biomolecules & Therapeutics 2023;31:682~691 https://doi.org/10.4062/biomolther.2023.162
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