Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

Download original image
Fig. 4. Lico D affects apoptosis in EGF- or TPA-induced JB6 cells. (A) The representative images of flow cytometry analysis in EGF- or TPA- induced JB6 cells treated with LicoD (2.5, 5, 10 μM) for 48 h. (B, C) The proportion of cells by stained with Annexin V/propidium iodide was represented as bar graphs. (D, E) Representative Western blots of apoptosis-related proteins expression of Caspase-3, Caspase-7 and Bax in EGF- or TPA-induced JB6 cells. JB6 cells were treated with EGF or TPA with 0, 2.5, 5, and 10 μM LicoD. The cell lysates were performed to western blotting using Caspase-3, Caspase-7 and Bax antibodies. β-actin was used as a loading control. (F, G) Quantitation of band intensity from blots by densitometry. The relative expression of cleaved-Caspase-3, cleaved-Caspase-7 and Bax was shown after normalization against Caspase-3, Caspase-7 and actin, respectively. Significantly different at: #p<0.05 and ##p<0.01 compared to the control; *p<0.05, **p<0.01, and ***p<0.001 compared to group treated with EGF or TPA only.
Biomolecules & Therapeutics 2023;31:682~691
© Biomolecules & Therapeutics