Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 1. LicoD structure and its effects on cell viability on EGF- or TPA-induced cell proliferation. (A) The structure of LicoD, a natural com-pound isolated from licorice root, is shown. (B) Inhibition of EGF-induced JB6 cell proliferation by LicoD. EGF was treated at a concentration of 10 ng/mL, and various concentrations of LicoD were applied for 24 and 48 h. Statistical analysis was performed to evaluate the signifi-cance of the results, and any differences were considered statistically significant when p<0.05. (C) The morphological changes in EGF-induced cells by LicoD. The cells were treated with EGF (10 ng/mL) at various concentrations of LicoD (2.5, 5, 10 μM) in serum-free MEM for 48 h. (D) MK2206 inhibited EGF-induced JB6 cell proliferation. EGF (10ng/mL) and MK2206 (0, 1, 3, and 10 μM) were treated and incubated for 24 and 48 h. (E) The effect of MK2206 on morphological changes in EGF-induced transformed cells. JB6 cells were treated with EGF with 0, 1, 3, and 10 μM MK2206 and then incubated for 48 h. The morphological changes in the cells were observed using a microscope. Statistical analysis was performed to evaluate the significance of the results, and significantly different at: ###p<0.001 compared to the control; ***p<0.001 compared to group treated with EGF only.
Biomolecules & Therapeutics 2023;31:682~691
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