Fig. 7. LCH increases multi-caspases activity HCT116 and HCT116-OxR cells. (A) Cells were treated with LCH (0, 4, 8 and 12 µM) for 48 h and stained with a Muse® MultiCaspase Kit, then the activity were measured using a Muse® Cell Analyzer. (B) The histogram of total caspase+ cells percentage. (C) Cells were pre-treated with pan-caspase inhibitor, Z-VAD-FMK (4 µM) for 3 h and then treated with or without 12 µM of LCH for 48 h. Cell viability was measured by the MTT assay. Results are presented as mean ± SD. *p<0.05, **p<0.01, and ***p<0.001 compared to the control group. ###p<0.001 compared to the cells treated with LCH only.
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