Fig. 5. LCH induces ROS production in HCT116 and HCT116-OxR CRC cells. (A) ROS generation by LCH treatment (0, 4, 8, and 12 μM) was measured using a Muse® Cell Analyzer. M1 and M2 represent ROS-negative and ROS-positive cell populations, respectively. Based on the bar in the graph, the left represents ROS-negative and the right represents ROS-positive. (B-D) Cells were pre-treated with NAC (4 mM) or vehicle for 3 h, then incubated with LCH (12 μM) for 48 h. (B) Cell viability was measured using the MTT assay. (C) Apoptosis was analyzed by flowcytometry. (D) Multi-caspase activity was analyzed using flowcytometry. Data are expressed as mean ± SD (*p<0.05 and ***p<0.001). ##p<0.01 and ###p<0.001 compared to the cells treated with LCH only.
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