Fig. 2. LCH induces sub-G1 population and arrests G1 phase of cell cycle in HCT116 and HCT116-OxR CRC cells. Cells were treated with indicated concentrations of LCH for 48 h. (A) Cells were analyzed by the Muse® Cell Analyzer to determine cell cycle distributions. (B) Representative histogram of the sub-G1 population. (C-D) Representative histograms of the cell cycle distributions in HCT116 and HCT116-OxR cells. Data are presented as the mean ± SD. *p<0.05, **p<0.01, or ***p<0.001 compared to the controls. (E) Cell lysates were examined for the expression of G1 phase-related proteins by Western blotting against antibodies, p21, p27, cyclin D1, CDK6, and CDK4. Actin was used as the loading control.
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