Fig. 1. LCH suppresses proliferation and induces apoptosis of HCT116 and HCT116-OxR CRC cells. (A) Cell viability was examined by MTT assay. HCT116, HCT116-OxR, HaCaT and JB6 cells were treated with 0, 4, 8, and 12 μM of LCH or 2 μM of Ox for 24 and 48 h. (B-D) Anchorage-independent colony growth was determined by soft agar assay in HCT116 and HCT116-OxR CRC cells treated with 0, 4, 8, and 12 μM of LCH or 2 μM of Ox for 14 days. Colonies were captured and counted under microscope. (B) Representative pictures. (C) Histogram of colonies number. (D) Histogram of over 50 μm diameter size. (E-F) Cells were treated with or without LCH at 4, 8 and 12 µM for 48 h. Flow cytometric analysis was performed using annexin V and 7-aminoactionomycin D (7-AAD) staining in CRC cells. (E) The results of flow cytometric analysis. (F) A histogram of annexin V-FITC/7-AAD stained cell population (%). Total apoptotic cells included Annexin V positive/7-AAD negative cells and Annexin V positive/7-AAD positive cells. All data are expressed as mean ± SD. *p<0.05, **p<0.01, and ***p<0.001.
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