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Fig. 2. Ratios of NRF2 nuclear translocation and NQO-1 expression are further reduced in AQP3-knockdown keratinocytes after H2O2 treatment. (A-C) Representative western blotting using anti-AQP3 (A), anti-NRF2 (B), and anti-NQO-1 antibodies (C) in cultured keratinocytes (total cell extracts or nuclear fractions), with or without AQP3 knockdown in the presence or absence of H2O2 treatment. β-actin was used as an internal control. The data represent the means ± SD from four independent experiments. *p<0.05 vs control keratinocytes [con (-)], #p<0.05 vs relative ratios in AQP3-knockdown keratinocytes [AQP3 (-)] without H2O2 treatment.
Biomolecules & Therapeutics 2023;31:648~654
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