Fig. 8. Effect of HAPLN1 siRNA on SIRT1 levels. (A, B) The protein levels of SIRT1, Ac-p53, p21CIP1, and p16INK4a in the various groups were determined by western blot analysis. (C) The levels of HAPLN1 mRNA in young HUVECs 24 h after transfection with control siRNA and siRNA against HAPLN1. Determination of SIRT1, p53, p21CIP1, and p16INK4a mRNA levels in HUVECs transfected with control siRNA or siRNA against HAPLN1. *p<0.05; or **p<0.005; and ***p<0.0005 versus control siRNA treatment (n=3 per group).
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