Fig. 4. Roles of rhHAPLN1 in SA-β-gal activity. (A) Young HUVECs were cultured with 2.5 ng/mL rhHAPLN1 and/or 5 ng/mL IL-17A for 24 h and then stained for SA-β-gal. (B) Senescent HUVECs were cultured with 2.5 ng/mL rhHAPLN1 for 24 h and SA-β-gal staining was performed. Cells stained blue-green, indicating SA-β-gal activity. Scale bar=100 μm. #p<0.05 versus Control (young HUVECs). *p<0.05, **p<0.005 versus IL-17A treatment or Control (senescent HUVECs, n=3 per group).
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