Fig. 2. HUVECs senescence markers. (A) The protein levels of IL-17A, Ac-p53, p21CIP1, and p16INK4a in the various groups were determined by western blot analysis. (B) The mRNA levels of IL-17A, p53, p21CIP1, and p16INK4a in the various groups were measured by qRT-PCR. (C, D, E) The protein levels of eNOS, p-eNOS, SIRT1, Cyclin E, and CDK2 in various groups were determined by western blot analysis. (F) Cell cycle progression was assessed by flow cytometric analysis. The percentage of cells in G1 and S/G2 phases was quantified in young and senescent HUVECs. **p<0.005; and ****p<0.0001 versus young HUVECs (n=3 per group).
© Biomolecules & Therapeutics
