Fig. 1. Characterization of senescent HUVECs. (A) Representative images of young and senescent HUVECs stained for SA-β-gal are shown. Cells stained blue-green, indicating SA-β-gal activity. (B) Percentage of migrated area by HUVECs in young and senescent groups. (C) Quantification of the number of meshes, branching intervals and total tube length by HUVECs in young and senescent groups. Scale bar=100 μm. *p<0.05; or **p<0.005; ***p<0.0005; and ****p<0.0001 versus young HUVECs (n=3 per group).
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