Fig. 5. Renoprotective effects of melatonin and zileuton in the UUO mouse model. (A) Expression levels of ferroptosis-related proteins (4-HNE, 5-LOX, and GPX4) in kidney tissue were determined by immunohistochemistry. (B) Expression levels of fibrosis-related proteins including α-SMA and fibronectin in kidney tissue were confirmed by immunohistochemistry and Sirius red staining. (C) Expression levels of AKT/mTOR/NRF2 signaling pathway-related proteins in kidney tissue were determined by immunohistochemistry. Data are presented as means ± SD (*p<0.05, **p<0.01 and ***p<0.001). Scale bar=50 μm.
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