Fig. 4. Kidney injury inhibition by the combination of melatonin and zileuton in the UUO mouse model. (A) Mice were intraperitoneally administered 20 mg/kg melatonin and 20 mg/kg zileuton, once per day for one week (n=10). (B) Histological analysis of UUO mice model tissues based by hematoxylin and eosin (H&E) staining; tubular injury scoring: score 0: normal, score 1: <10%, score 2: 10-25%, score 3: 26-50%, score4: 51-75% and score 5: >75% and glomerular size measurement using the ImageJ 1.41. (C) Drug toxicity was assessed by body weight, serum AST concentration, and liver histological analysis (n=8). Scale bar=50 μm. Data are presented as means ± SD (*p<0.05, **p<0.01 and ***p<0.001).
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