Fig. 7. SC downregulated key proteins and kinases in the TLR4-TRAF6-MAPK pathway in LPS and IFN-γ-stimulated RAW 264.7 cells (n=3). (A) Cells were cultured in T75 cell flasks and were pre-treated with S, C or SC 1 h prior to LPS (1 μg/mL) for 24 h. (B) Protein expression levels of TLR4 and TRAF-6 were analyzed by Western blot using whole protein extracts. (C) Cells were cultured in T75 cell flasks and were pre-treated with S, C or SC 1 h prior to LPS (1 μg/mL) for 40 min. (D) Protein expression levels of phosphorylated JNK (p-JNK), JNK, p-cJun, and cJun were analyzed by Western blot using whole protein extracts. All results (n=3) were expressed as the mean ± SEM, #p<0.05 vs. blank control; *p<0.05, **p<0.01, ****p<0.0001 vs. LPS-stimulated cells.
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