Fig. 6. The impact of Nrf2 blockage on the action of SC in inhibiting NFκB translocation. (A) RAW 264.7 cells were co-incubated with medium (Blank), SC (5 µg/mL), Bru (50 nM), SC (5 µg/mL) + Bru (50 nM) for 2 h prior to the stimulation of LPS (1 µg/mL) for another 30 min. Then the cells were prepared and subjected to fluorescent staining. Images were taken by using a confocal microscope with 40× magnification. Blue: DAPI in the nucleus, green: NFκB p65 in the RAW 264.7 cells. Scale bar=20 µm. Data points represent mean ± standard deviation from analysis of 3 separate images. (B) Data points represent mean ± standard deviation from analysis of 3 separate images. ###p<0.001 vs. blank control; *p<0.05 vs. LPS-stimulated cells, &&&&p<0.0001 vs. LPS+Bru.
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