Fig. 5. SC reduced NFκB translocation and microRNA-155-5p expressions in LPS and IFN-γ-stimulated RAW 264.7 cells (n=3 individual experiments). (A) S, C, and SC at 5 µg/mL inhibited LPS activated NFκB p65 translocation in the RAW 264.7 cells by immunofluorescence staining. Images were taken by using a confocal microscope with 60x magnification. Blue: DAPI in the nucleus, red: NFκB p65 in the RAW 264.7 cells. Scale bar=20 µm. (B) Data points represent mean ± standard deviation from analysis of 3 separate images. #p<0.05 vs. blank control; ****p<0.0001 vs. LPS-stimulated cells, &&p<0.01, &&&&p<0.0001 vs. S or C. (C) Cells were cultured in T25 cell flasks and were pre-treated with S, C and SC (2.5 µg/mL) 1 h prior to LPS (1 µg/mL) for 24 h. The samples were then subjected to real-time PCR after RNA collection and cDNA synthesis. The mmu-miR-155-5p fold changes compared to untreated cells (Blank control). The data was expressed as the mean ± SEM. #p<0.05 vs. blank control; *p<0.05, ***p<0.001 vs. LPS stimulation.
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