Fig. 4. SC synergistically induced Nrf2 activation as detected by luciferase assay. Dose-response curves of SC (A), C (B), S (C) and tBHQ (D) on Nrf2 activation and cell viability using AREc32 cells. All the data are presented as mean with SEM (n≥3 individual experiments). CI-Fa curve (E) and isobologram (F) revealed strong synergistic activity of SC in activating Nf2 total protein. CI<1 represents synergy. Fa represents the Nrf2 induction effect. (G) Western blot analysis of HO-1 total protein expression. RAW 264.7 cells were treated with medium only (Blank), S (10 µg/mL), C (10 µg/mL), and SC (10 µg/mL) for 24 h, and the total protein was lysed for Western blotting. *p<0.05 vs. Blank. (H) Western blot analysis of HO-1 total protein expression with the co-incubation of Nrf2 blockage (Bru). RAW 264.7 cells were treated with medium only (Blank), SC (5 µg/mL), Bru (50 nM), and SC (5 µg/mL)+Bru (50 nM) for 24 h, and the total protein was lysed for Western blotting. *p<0.05, **p<0.01 vs. Blank. #p<0.05 vs. Bru.
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