Fig. 2. SC showed a broader and stronger proinflammatory inhibition in cytokine microarray in human THP-1 cells. THP-1 cells (differentiated by 50 nM PMA) were treated with media, S (2.5 µg/mL), C (2.5 µg/mL) and SC (2.5 µg/mL), and stimulated with LPS (1 µg/mL) and IFN-ɣ (50 ng/mL) for 24 h. Four membranes were used to show activated cytokines treated by LPS and IFN- ɣ, pre-treated with S, C and SC (A). The list of activated cytokines is shown in (B). The semi-quantitative results are shown as averaged IntDen (n=2, each cytokine has two replicated dots) analysed by ImageJ (C). *p<0.05, **p<0.01, ***p<0.001 vs. LPS, #p< 0.05, ##p<0.01, ###p<0.001, ####p<0.0001 vs. S or C.
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