Fig. 1. SC synergistically reduced LPS and IFN-γ-induced TNF and IL-6 productions in murine RAW 264.7 and PMA-differentiated human THP-1 cell lines (n≥3 individual experiments). (A) Cell viability of RAW 264.7 cells treated by S, C and SC (0-22.10 µg/mL). (B) Cell viability of THP-1 cells treated by S, C and SC (0-22.10 µg/mL). Dose-response curves of S, C and SC in inhibiting TNF (C) and CI-Fa curve (D) in RAW 264.7 cells, and in THP-1 cells (E, F). Dose-response curves of S, C and SC in inhibiting IL-6 (G) and CI-Fa curve (H) in RAW 264.7 cells, and in THP-1 cells (I, J). The CI-Fa curves revealed the interaction (synergistic when CI<1 or antagonistic when CI>1) of SC at all TNF and IL-6 inhibitory levels (Fa values ranged from 0 to 1).
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