Fig. 3. Effects of 5-HT2AR endocytosis on ERK activation. (A) HEK-293 cells were transfected with 2 μg HA-tagged 5-HT2AR cDNA in pRC/CMV together with 4 μg WT-dynamin2 or K44A-dynamin2 constructs in pCMV5. Cells were treated with 1 μM 5-HT for 15 min. ***p<0.001 compared to the WT-dynamin2 group (n=3). (B) HEK-293 cells were transfected as mentioned above. Cells were treated with 1 μM 5-HT for 5 min. **p<0.01 compared to the WT-dynamin2 group (n=3).
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