Fig. 6. Melatonin inhibited the apoptosis of renal proximal tubular epithelial cells induced by AA in vivo and in vitro. (A) TUNEL staining in kidney sections of mice (scale bars: 20 µm). (B) The statistic result of the percentage of TUNEL positive cells in kidney sections of mice (n=6). (C) TUNEL staining in NRK-52E and HK-2 cells after melatonin (1 mM) treatment under AA (2.5 µg/mL) exposure (scale bars: 20 µm). (D) The statistical result of the percentage of TUNEL positive cells in NRK-52E and HK-2 cells (n=3). (E) Western blot analysis of caspase-3 and cleaved caspase-3 expression. (F) The statistic result of relative expression of cleaved caspase-3 to GAPDH (n=3). CONT means control. Mel means melatonin. Values are means ± SEM, *p<0.05, **p<0.01, ***p<0.001.
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