Fig. 5. Melatonin prevented the autophagy of renal proximal tubular epithelial cells induced AA. (A) Immunofluorescent detection of LC3A/B expression in NRK-52E and HK-2 cells after melatonin (1 mM) treatment after exposure AA (2.5 µg/mL) (scale bars: 20 µm). Cell nuclei were stained with DAPI (blue). (B) The statistic result of LC3A/B expression. (C) Western blot analysis of LC3A/B-I/II and P62 expression. (D, E) The statistic result of LC3A/B-I/II and P62 expression to GAPDH. CONT means control. Mel means melatonin. Values are means ± SEM, *p<0.05, **p<0.01, ***p<0.001, n=3.
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