Fig. 3. Melatonin repressed the oxidative stress induced by AA in vivo and in vitro. (A) ROS detection using DHE probe in kidney tissues of mice (n=6). (B) SOD detection in serum of mice (n=6). (C) MDA detection in serum of mice (n=6). (D) DCFD-DA immunofluorescence detection of intracellular ROS levels in NRK-52E and HK-2 cells after melatonin (1mM) treatment in response to AA (2.5 µg/mL) (scale bars: 50 μm). (E) The statistic result of relative DCFH-DA fluorescence intensity in NRK-52E and HK-2 cells (n=3). (F) Western blot analysis of iNOS expression in NRK-52E and HK-2 cells. (G) The statistic result of relative expression of iNOS to GAPDH. CONT means control. Mel means melatonin. Values are means ± SEM, *p<0.05, **p<0.01, ***p<0.001.
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