Fig. 2. Melatonin inhibited the cytotoxicity induced by AA in renal proximal tubular epithelial cells. (A, B) MTT assay of NRK-52E and HK-2 cells after AA exposure at different concentrations (0.5, 2.5, 5, 10 µg/mL) and different time points (24, 48, 72 h). (C) MTT assay of NRK-52E and HK-2 cells after melatonin (1 mM) treatment in response to AA (2.5 µg/mL). (D) HE staining of NRK-52E and HK-2 cells (scale bars: 50 µm). (E) Colony formation assay. (F) The statistic result of colony formation rate. CONT means control. Mel means melatonin. Values are means ± SEM, *p<0.05, **p<0.01, ***p<0.001, n=3.
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