Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 5. HAS2 overexpression increased NRF2 signaling. (A) HAS2 was overexpressed in MCF7 (HAS2-ov) cells and relative HAS2 transcript levels were determined by relative quantification real-time PCR analysis. HPRT1 was used as a housekeeping control gene. Data represent ratios with respect to negative control (NC) and are reported as the mean ± SD of two experiments. (B) HA concentration was measured in negative control (NC) and HAS2-overexpressing MCF7 (HAS2-ov) cells using ELISA. Values represent the mean ± SD from four sampled wells. ap<0.05 compared with NC. (C) CD44, NRF2, AKR1C1, and MDR1 protein levels were monitored in NC and HAS2-overexpressing MCF7 (HAS2-ov) cells. (D) Conditioned medium (CM) was harvested from cultured HAS2-ov cells, and then added into MCF7-DR cells and cultured for 24 h. NRF2 protein levels were monitored in the CM-treated or normal medium group (CTRL). (E) Cell viability was monitored after doxorubicin (1 and 4 μM) incubation in CTRL and CM-treated DR cells. Values represent the mean ± SD from 8 sampled wells. ap<0.05 compared with CTRL. In the case of western blot results, similar blots were obtained in at least three experiments.
Biomolecules & Therapeutics 2022;30:368~379 https://doi.org/10.4062/biomolther.2022.074
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