Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 2. MCF7-DR cells show activated NRF2 signaling. (A) NRF2 and aldo-keto reductase 1C1 (AKR1C1) transcript levels were monitored in MCF7 and DR cells using relative quantification real-time PCR analysis. Hypoxanthine phosphoribosyltransferase-1 (HPRT1) was used as a housekeeping control gene. Data represent ratios with respect to MCF7 and are reported as mean ± SD of three experiments. (B) MDR1, MRP1, MRP2, and BCRP transcript levels were monitored in MCF7 and DR cells by relative quantification of real-time PCR analysis. HPRT1 was used as a housekeeping control gene. Data represent ratios with respect to MCF7 cells and are reported as the mean ± SD of three experiments. (C) NRF2, AKR1C1, and multidrug resistance 1 (MDR1) protein levels were determined using western blotting analysis. (D) Cell viability was monitored after doxorubicin incubation for 72 h in the non-specific control RNA-transfected (siCTRL) or NRF2-specific siRNA-transfected (siNRF2) DR cells. Values represent the mean ± SD from four sampled wells. ap<0.05 compared with the siCTRL. (E) DR cells were treated with HA (25 or 50 μg/mL) for 24 h, and CD44, NRF2, AKR1C1, and MDR1 protein levels were monitored using western blotting analysis. In the case of western blot results, similar blots were obtained in at least three experiments.
Biomolecules & Therapeutics 2022;30:368~379 https://doi.org/10.4062/biomolther.2022.074
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