Fig. 1. Doxorubicin-resistant breast cancer MCF7 (MCF7-DR) cells show high levels of hyaluronic acid (HA) through increased expression of HA synthase (HAS)-2. (A) Cluster of differentiation (CD)-44 protein levels were determined in MCF7 and MCF7-DR (DR) cells by western blotting analysis. (B) CD44 expression levels were determined in MCF7 and DR cells using flow cytometry analysis. (C) HA concentration was measured in MCF7 and DR cells by enzyme-linked immunosorbent assay (ELISA) assay. Values represent the mean ± standard deviation (SD) from four experiments. ap<0.05 compared with MCF7 cells. (D) HAS1, HAS2, and HAS3 transcript levels were monitored in MCF7 and DR cells using real-time polymerase chain reaction (qPCR). Values represent the mean ± SD from three experiments. ap<0.05 compared with MCF7 cells. N.D, not determined. (E) HAS2 protein levels were assessed in MCF7 and DR cells using western blotting analysis. (F) Immunocytochemistry analysis using HAS2 antibody was performed in MCF7 and DR cells. In the case of western blotting results, similar blots were obtained in at least three experiments.
© Biomolecules & Therapeutics
