Fig. 9. Inhibition of different stages of autophagy and its effect on the cytotoxicity of BITC in AGS cells. AGS cells were treated with BITC for 24 h after 2-h pre-treatment with (A) 0.5 µM wortmannin and (B) 100 nM bafilomycin A1. Then, the cell viability was measured by MTT assay. Data were calculated from at least three independent experiments and expressed as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 versus the control, and #p<0.05 versus BITC treatment alone.
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