Fig. 7. BITC reduced the expression level of mature cathepsins D, L, and B in AGS cells. (A) AGS cells were treated with 5, 10, or 15 µM BITC for 24 h. Whole cell lysate was prepared, and the cathepsin D protein level was determined by western blotting. Under the same conditions as above, (B) cathepsin L and (C) cathepsin B protein levels were determined by western blotting. β-actin or GAPDH was used as a loading control. Data were calculated from three independent experiments and expressed as the mean ± SEM. *p<0.05, **p<0.01 and ***p<0.001 versus the control.
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