Fig. 6. BITC increased the expression level of lysosomal membrane protein LAMP2 in AGS cells. (A) AGS cells were treated with 5, 10, or 15 µM BITC for 24 h. Whole cell lysate was prepared, and the LAMP2 protein level was measured by western blotting. β-actin was used as a loading control. (B) AGS cells were treated with 5, 10, or 15 µM BITC for 7 h, incubated with anti-LAMP2 antibody, and was detected via Alexa Flour 488-conjugated secondary antibody (green) by a confocal microscope (scale-bar=50 μm). DAPI (blue) was used for nuclear staining. Data were calculated from three independent experiments and expressed as the mean ± SEM. **p<0.01 versus the control.
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