Fig. 3. Effect of BITC on other autophagy markers: Beclin1 and Atg5-Atg12 protein expression and mTOR phosphorylation in AGS cells. AGS cells were treated with 5, 10, or 15 µM BITC for 24 h. Whole cell lysate was prepared, and Beclin1, Atg5-Atg12, and p-mTOR/mTOR levels were determined by western blotting. β-actin was used as a loading control. Data were calculated from three independent experiments and expressed as the mean ± SEM. ***p<0.001 versus the control.
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