Fig. 2. BITC increased the expression levels of LC3B and p62 proteins AGS cells. (A) AGS cells were treated with 10 µM BITC for 8, 12, or 24 h. Whole cell lysate was prepared, and LC3B and p62 levels were determined by western blotting. β-actin was used as a loading control. (B) AGS cells were treated with 5, 10, or 15 µM BITC for 24 h. Whole cell lysate was prepared, and LC3B and p62 levels were determined by western blotting. β-actin was used as a loading control. (C) AGS cells were treated with 5, 10, or 15 μM BITC for 12 h and mRNA level was assessed by q-PCR. Data were calculated from three independent experiments and expressed as the mean ± SEM. *p<0.05, **p<0.01, and ***p<0.001 versus the control.
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