Fig. 10. No relationship between BITC-induced LC3 B accumulation and cytotoxicity. (A) AGS cells were transfected with 50 nM LC3B siRNA or control siRNA for 12 h and incubated with 10 µM BITC for 8 h. Whole cell lysate was prepared, and siRNA efficacy was confirmed by detecting the LC3B protein expression via western blotting. β-actin was used as a loading control. (B) AGS cells were transfected with 50 nM LC3B siRNA or control siRNA for 12 h, followed by 10 µM BITC treatment for 24 h. Cell viability changes were evaluated by MTT assay. Data were calculated from three independent experiments and expressed as the mean ± SEM. *p<0.05, **p<0.01 versus the control.
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