Fig. 1. BITC inhibited AGS cell viability in time and concentration-dependent manners and induced apoptotic features. (A) AGS cells were treated with BITC 2.5, 5, 10, 20, or 40 µM for 24 or 48 h. Then an MTT assay was conducted to measure the cell viability. (B) 6-h BITC-treated AGS cells were visualized under a fluorescence microscope to examine morphological changes (scale-bar=50 μm). (C) 24-h BITC-treated AGS cells were stained with a nuclear dye DAPI (blue) and nuclear condensation was visualized by a fluorescence microscope (scale-bar=50 μm). Data were calculated from three independent experiments and expressed as the mean ± SEM. *p<0.05, **p<0.01, and ***p<0.001 versus the control.
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