Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 2. PRR16 promotes epithelial mesenchymal metastasis in breast cancer cells. (A) Changes in the cell shape in response to PRR16 expression. (B) Effect of PRR16 expression on EMT markers. (C) Effect of PRR16 expression on EMT regulating transcription factors. (D) Confocal image demonstrating the influence of PRR16 expression on EMT markers. (E) Effect of PRR16 expression on cell motility and invasiveness. MCF7 and SK-BR-3 cells were transfected with plasmid DNA to overexpress PRR16, while MDA-MB-231 cells were transfected with siRNA to decrease PRR16 expression. The protein level of the EMT markers and transcription factors were validated by Western blot, and the mRNA levels of the EMT marker were confirmed using PCR. Confocal microscopic analysis validated the protein levels of the EMT markers. Cell migration and invasiveness were assessed using the fibronectin-coated transwell assay and Matrigel-coated transwell assays, respectively. Data are reported as the mean ± SD of three to four independent experiments. ***p<0.001 when compared between the indicated groups using a Student’s t-test.
Biomolecules & Therapeutics 2022;30:340~347 https://doi.org/10.4062/biomolther.2022.066
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