Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 4. Effect of urushiol V on AMPK/mTOR signaling pathway. (A) SW480 cells were pre-treated with CHX for 2 h and then further treated with urushiol V for 16 h. FoxM1 protein was detected by Western blotting. Data are shown as the mean ± SD of three independent experiments. ***p<0.001 as compared to vehicle control; ###p<0.001 as compared to treated with 10 μg/mL CHX. (B) SW480 cells were pre-treated with MG132 or CQ for 2 h and then further treated with urushiol V for 16 h. FoxM1 protein was detected by Western blotting. Data are shown as the mean ± SD of three independent experiments. **p<0.01, ***p<0.001 as compared to vehicle control; ###p<0.001 as compared to treated with 10 μM MG132 or 20 μM CQ. (C) SW480 cells were treated with indicated concentrations of urushiol V for 24 h, and protein levels of phospho-AMPK and phospho-mTOR were detected by Western blotting. Data are shown as the mean ± SD of three independent experiments. **p<0.01, ***p<0.001 as compared to vehicle control.
Biomolecules & Therapeutics 2022;30:257~264 https://doi.org/10.4062/biomolther.2022.008
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