Fig. 7. BT inhibits the secretion of inflammatory cytokines by blocking P2X7r and NLRP3 expression in chronic EtOH-treated mice. (A) Protein expression of IL-1β was determined by ELISA kit. (B) Protein expression of IL-6 was determined by ELISA kit. (C) Representative Western blotting analysis for expressions of IL-6, IL-18, IL-1α, IL-1β and Caspase-1. (D) Representative RT-PCR analysis for expressions of IL-6, IL-18, IL-1α, IL-1β, Caspase-1 and TNF-α. Densitometric values were normalized against GAPDH. ###p<0.001, ##p<0.01 vs normal group, *p<0.05, **p<0.01, ***p<0.001 vs EtOH group. All histograms represent the mean ± SD of at least three independent assays.
© Biomolecules & Therapeutics
