Fig. 6. BT regulates P2X7r and NLRP3 expressions in chronic EtOH administration mice. (A) Representative Western blotting analysis for expressions of P2X7r and NLRP3. (B) Representative RT-PCR analysis for expressions of P2X7r and NLRP3. (C) Immunohistochemical staining of P2X7r present in 100× and 400× magnification. (D) Immunofluorescence staining of P2X7r present in 100× magnification and immunofluorescence staining of NLRP3 present in 100× magnification. Densitometric values were normalized against GAPDH. ###p<0.001 vs normal group, ***p<0.001 vs EtOH group. All histograms represent the mean ± SD of at least three independent assays.
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